About the training
There have been a number of major advances in molecular biology in the past few years. The techniques of molecular biology have provided new tools for research and disease diagnosis. They have been used routinely since the middle of 1980s. New technologies either improve existing techniques or help development of new approaches to explore solutions to old problems in order to generate information more quickly, easily, accurately or in a more easily repeatable fashion than the existing methods.
Molecular methods offer considerable advantage over the conventional methods, and they are applicable in diverse areas of research and diagnosis of infectious diseases. Following introduction of nucleic acid amplification and sequencing techniques, several nucleic acid-based molecular techniques have been developed which are applicable in diverse areas of biology, viz. identification and differentiation of species, typing and sub-typing of microbes for molecular epidemiological study, diagnosis of infectious diseases, understanding cancer biology, forensic sciences, etc. The major advantages of using molecular methods include their rapidity, accuracy, reliability and requirement of a very minute quantity of samples for processing.
The present training programme is proposed with a view to train selected teachers, researchers and biomedical practitioners on application of advanced tools and techniques in molecular biology specifically giving hands-on experience on cloning and expression as well as basics of NGS data analysis so that they may effectively use them in their study and research for the ultimate benefit of the masses. The participants are advised to bring their own laptop for a better experience of the sequence analysis practicals.
Contents:
- DNA extraction from bacteria and animal tissues
- Polymerase chain reaction and gel electrophoresis
- Plasmid isolation
- Molecular cloning and expression
- Primer designing for PCR
- NGS data analysis
- Last date of application: 5th July, 2022