About the training



Recent advancements in molecular biology have made significant impact on every field of biological research. Several molecular techniques with extensively high level of sensitivity and specificity have been developed during the last three decades which have made detection and typing of microbes including human and animal pathogens very rapid, accurate and cost-effective. Most of these advanced techniques are based on detection and identification of specific molecular signatures of the organisms in their genome, either DNA or RNA.

Molecular typing is an important tool today for surveillance and monitoring the prevalence of certain strains of a pathogen and its mutants in different epidemiological situations. It provides highly discriminatory information on clonal and strain variations, origins, and ecology of the isolates when used in conjunction with epidemiological data during disease outbreaks.

The Advanced Level State Biotech Hub, Assam established in Department of Animal Biotechnology, College of Veterinary Science, AAU, Khanapara, Guwahati is well-equipped to undertake research on relevant areas of application of molecular tools for detection and confirmatory identification of bacterial and viral pathogens as well as other microbes. Various molecular typing methods, viz., random amplified polymorphic DNA typing (RAPD), Restriction Fragment Length Polymorphism (RFLP), enterobacterial repetitive intergenic consensus typing (ERIC-PCR), repetitive extragenic palindromic element typing (REP-PCR), Pulse Field Gel Electrophoresis (PFGE), Multi Locus Sequence Typing (MLST), Plasmid Profiling are routinely being used in the lab for typing of animal and human pathogens. Real Time PCR-based molecular detection is also currently considered as the gold standard for the molecular detection of many pathogens in the field of disease diagnosis.

In this proposed training, the selected participants will be offered hands-on training on various molecular techniques and typing methods like conventional PCR, reverse transcription PCR, RAPD, RFLP, PAGE, rep-PCR, Plasmid Profiling and Real-Time PCR-based molecular detection of bacteria and viruses. The hands-on sessions will be supported by theoretical lectures on relevant topics to be delivered by internal and external experts to give a fair exposure to the participants.

Contents:

  1. DNA extraction from bacteria, mammalian as well as plant tissues

  2. RNA isolation from whole blood

  3. PCR and gel electrophoresis

  4. Polyacrylamide gel electrophoresis (PAGE)

  5. Reverse transcription PCR

  6. Real-time PCR

  7. Plasmid profiling

  8. RAPD

  9. RFLP

  10. Rep-PCR

  11. PCR primer designing

  12. Sequence analysis and editing

  13. Molecular phylogeny and phylogenetic analysis

  • Last date of application: 8th November, 2022