About the training



There have been a number of major advances in molecular biology in the past few years. The techniques of molecular biology present new tools for research and diagnosis. New technologies either improve existing techniques or provide new approaches to find solutions to old problems in order to generate information more quickly, easily, accurately or in a more easily repeatable fashion than the existing ones.

Molecular methods offer considerable advantage over the conventional ones applied to biomedical sciences with their wide application in diverse areas of biological research and for diagnosis of infectious diseases. Following introduction of nucleic acid amplification and sequencing techniques, several nucleic acid-based molecular techniques have been developed which are applicable in diverse areas of biology, viz. identification and differentiation of species, typing and sub-typing of microbes for molecular epidemiological study, diagnosis of infectious diseases, understanding cancer biology, forensic sciences, etc. The major advantages of using molecular methods include their rapidity, accuracy, reliability and requirement of a very minute quantity of samples for processing.

This training programme has been proposed with a view to train selected teachers, researchers and biomedical practitioners on application of advanced tools and techniques in molecular biology specifically giving hands on experience on molecular cloning as well as expression and purification of recombinant protein so that they may effectively use them in their own research for the ultimate benefit of the masses. The selected candidates are advised to bring own laptop for a better experience of the sequence analysis practicals.

Contents:

  1. DNA extraction from bacteria and animal tissues

  2. Polymerase chain reaction and gel electrophoresis

  3. Plasmid isolation

  4. Various steps of molecular cloning and expression.

  5. Protein purification

  6. Primer designing for PCR

  • Last date of application: 17th February, 2023