About the training
Cell culture, the art of growing cells outside their natural environment, has revolutionized our understanding of biology. From fundamental research to groundbreaking medical advancements, this seemingly simple technique has become a cornerstone of modern science. The impact of cell culture extends far beyond the research laboratory. Cell culture has been instrumental in various aspects of life science research with practical applications like production of different vaccines and biopharmaceuticals, stem cell therapy and tissue engineering for regenerative medicines, understanding disease mechanisms, drug discovery and development, genetic engineering and gene therapy, environmental monitoring by assessing the toxicity of environmental pollutants etc. Advanced Level State Biotech Hub, Assam hosted in the Department of Animal Biotechnology, College of Veterinary Science, Khanapara is well equipped to undertake the basic to advance research on animal cell culture techniques. This training will be organized with an aim to train 12 selected teachers/ researchers/ biomedical workers / veterinary or medical research scholars on applications of animal cell culture techniques in basic and advanced research and expression of heterologous protein in mammalian cell lines specifically giving hands-on experiences in these aspects. The training will not only merely help to develop technical skills but also provide opportunities in future academic and research career and prospects for collaboration.
Contents of the hands-on training
Theory lectures: Introduction to animal cell culture and its application, basic requirements for animal cells and different culture techniques, Stem cells in regenerative medicine, Strategies for gene cloning and eukaryotic expression, Heterologous Gene expression in mammalian cell lines and purification of recombinant proteins, Virus isolation in animal cell culture and their titration, Cell viability, proliferation and cytotoxicity assays.
Practical sessions: Sterilization procedures for cell culture work, Preparation of cell culture media and reagents, Handling/ subculture and maintenance of cell lines, Cell counting and cryopreservation of cell lines, Primary culture establishment from Chicken Embryo, Ligation of gene of interest to expression vector and transformation to competent cells, Selection and screening of clones by colony PCR, Extraction of Plasmid and evaluation of its quality and concentration, Transfection of mammalian cell line for protein expression, Detection of expressed protein by GFP/RFP marker and Dot Blot assay, Isolation of virus in animal cell culture, Extraction of Total DNA/RNA from mammalian cells and determination of quality and concentration, Observation of CPE in cell culture and confirmation of virus isolates by PCR/ RT-PCR, Determination of cell proliferation and cytotoxicity by MTT assay, Harvesting and purification of secreted recombinant proteins from the transfected mammalian cells, Characterization of mammalian cell expressed protein by SDS-PAGE & western blotting.
- Last date of application: 14th June, 2024