About the training



Ever since its inception, the DBT-funded State Level Biotech Hub, College of Veterinary Science, Khanapara, Guwahati, has been thriving for development of skilled manpower in the niche areas of molecular biology and biotechnology in the North-Eastern Region besides its other activities related to education and research. In December, 2015, the hub was upgraded to an Advanced State Level Biotech Hub by DBT Govt. of India.

As a part of its initiatives, the hub has been regularly organizing training programmes in different areas of molecular biology and biotechnology for teachers, scientists, research scholars and students of the region. We are now happy to announce our next training programme in the area of animal cell culture and microbial typing from 12th - 16th February, 2018.

This training will cover both practical and relevant theoretical aspects of basic and molecular microbiology, more specifically molecular bacteriology, isolation of nucleic acids, typing of microbes using different methods, phylogenetic analysis, basic animal cell culture techniques and sequence analysis. Even though basic microbiological techniques like culture, staining, biochemical test etc. are the irreplaceable foundations in any microbiological method, most of the modern day advanced laboratory methods of microbiology rely substantially on different biotechnological tools such as PCR and its variants, RFLP, RAPD, REP-PCR etc to differentiate in between species of microbes at molecular level. Cell/tissue culture methods are also prominent cornerstones of modern biological research and have been able to bring about significant changes in regenerative medicine and fertility management in human and animals.

The present training will be a balanced amalgamation of both the theoretical and practical aspects of these two areas with more emphasis on hands-on learning. Experienced researchers from the host institute will help the participants to develop clear understanding and practical skills on different techniques as listed below.

Contents:

  1. Basic microbiological techniques

  2. Genomic DNA extraction from Bacterial Cells

  3. Quality and Quantity check of extracted DNA

  4. Conventional PCR & agarose gel electrophoresis

  5. RFLP& RAPD

  6. Rep-PCR

  7. Plasmid Isolation & profiling

  8. Automated rep-PCR (DiversiLab System)

  9. Basics of Animal Cell Culture

  10. Primary cell culture from Chicken Embryo

  11. Subculture, Cell Counting & Viability Testing

  12. Sequence Analysis

  13. Molecular Phylogeny and Phylogenetic analysis

  • Last date of application: 8th February, 2018